posted on 2023-09-25, 19:41authored byAlexis HrubyAlexis Hruby, I.A.M.A. Teixeira, P.S. Yoder, T. Pilonero, M.D. Hanigan
“Valine and non-essential amino acids affect bi-directional transport rates of leucine and isoleucine in bovine mammary epithelial cells”. This study assessed transport rates of 7 AA to evaluate the effects of high and low concentrations of Val and Ala, Gln, and Gly provided as a group in primary bovine mammary epithelial cells. Treatment effects on Leu and Ile were attributed to increased intracellular concentrations of non-essential AA used as exchange currency combined with the reduced shared transporter competition from Val. These results demonstrate that the efficiency of branched-chain amino acid uptake by mammary cells is affected by competition among the branched-chain AA and the synergistic effects of non-essential AA.
Virginia Agricultural Experiment Station
Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture
o Supplementary Figure 1. An example fit of the mean [15N]-isotope ratios for Asp, Ala, and Glu with respect to time after removal of [15N]-labeled media.
o Supplementary Figure 2. Example of observed and predicted [13C]-serine isotope ratios with respect to time after addition of the [13C]-labeled media polynomial fit of Ser over time.
o Observed concentrations of AA in acclimation media prior to cell exposure (µM).
o m/z of ions monitored during mass spectrometry to determine unlabeled and labeled amino acid concentrations in samples.
o Initial (i) bovine mammary epithelial cell pool sizes, and isotopic ratios for modeled amino acids. Data are least-squares means.
o Target total AA Concentration (µM) in the [15N]-labeled and [13C]-labeled media.
o Total amino acid concentrations (µM) of [15N]-labeled extracellular media every 8 h of the 24 h period.
o Evaluations of predictions of [15N]-Ala, Asp and Glu (IRnKAii), which are precursors for transamination and [13C]-Ser as a precursor for re-methylation of methionine (IRSeri).
o Fit statistics for predictions of pool sizes and isotope ratios averaged by amino acid and across treatments.
o Effect of treatment and time on extracellular free AA concentrations (µM ) after addition of [13C]-labeled treatment media.