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Valine and non-essential amino acids affect bi-directional transport rates of leucine and isoleucine in bovine mammary epithelial cells

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posted on 2023-08-28, 14:55 authored by Alexis WestonAlexis Weston, I.A.M.A. Teixeira, P.S. Yoder, T. Pilonero, M.D. Hanigan

Valine and non-essential amino acids affect bi-directional transport rates of leucine and isoleucine in bovine mammary epithelial cells”. This study assessed transport rates of 7 AA to evaluate the effects of high and low concentrations of Val and Ala, Gln, and Gly provided as a group in primary bovine mammary epithelial cells. Treatment effects on Leu and Ile were attributed to increased intracellular concentrations of non-essential AA used as exchange currency combined with the reduced shared transporter competition from Val. These results demonstrate that the efficiency of branched-chain amino acid uptake by mammary cells is affected by competition among the branched-chain AA and the synergistic effects of non-essential AA.

Funding

Virginia Agricultural Experiment Station

Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture

National Animal Nutrition Program

National Institute of Food and Agriculture

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Virginia Tech College of Agriculture and Life Sciences David R. and Margaret Lincicome Endowment

Balchem Corp.

History

Publisher

University Libraries, Virginia Tech

Location

Blacksburg, VA

Corresponding Author Name

Alexis Hruby-Weston

Corresponding Author E-mail Address

achruby@vt.edu

Files/Folders in Dataset and Description

[SupplementaryFigures.pdf] - Supplementary Figure 1. An example fit of the mean [15N]-isotope ratios for Asp, Ala, and Glu with respect to time after removal of [15N]-labeled media. Supplementary Figure 2. Example of observed and predicted [13C]-serine isotope ratios with respect to time after addition of the [13C]-labeled media polynomial fit of Ser over time. [SupplementaryTable1.csv] - Observed concentrations of AA in acclimation media prior to cell exposure (µM). [SupplementaryTable2.csv] - m/z of ions monitored during mass spectrometry to determine unlabeled and labeled amino acid concentrations in samples. [SupplementaryTable3.csv] - Initial (i) pool sizes, and isotopic ratios for modeled amino acids. Calculations are lsmeans by AA and treatment. [SupplenmentaryTable4.csv] - Target total AA Concentration (µM) in the [15N]-labeled and [13C]-labeled media. [SupplementaryTable5.csv] - Total amino acid concentrations (µM) of [15N]-labeled extracellular media every 8 h of the 24 h period. [SupplementaryTable6.csv] - Evaluations of predictions of [15N]-Ala, Asp and Glu (IRnKAii), which are precursors for transamination and [13C]-Ser as a precursor for re-methylation of methionine (IRSeri). [SupplementaryTable7.csv] - Fit statistics for predictions of pool sizes and isotope ratios averaged by amino acid and across treatments. [SupplementaryTable8.csv] - Effect of treatment and time on extracellular free AA concentrations (µM ) after addition of [13C]-labeled treatment media.

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    Virginia Polytechnic Institute and State University

    Categories

    Keywords

    amino acid transportMathematical Modelstable isotopemammary glandbovineAnimal Nutrition

    Licence

    CC0

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