Virginia Tech
3 files

Kale shelf-life study

posted on 2022-10-28, 10:19 authored by Auja BywaterAuja Bywater, Monica PonderMonica Ponder

Pathogenic Campylobacter jejuni and E. coli O157:H7 were inoculated onto the surface of kale stored in a controlled environment at  4°C, ± 0.15°C,  and average humidity of 95%, ± 1.13°C, over a 23-day period. (days 0, 1, 2, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21), inoculated kale was destructively sampled and the surviving bacteria determined by serial dilution and plating onto Tryptic soy agar, Charcoal cefoperozone deoxycholate agar, and Eosin methylene blue for total aerobic bacteria, Campylobacter and E. coli O157:H7, respectively.Enrichment and PCR were used for detection when pathogens were not detected using serial dilution and plating. Aerobic heterotrophic bacteria increased over the 23-day period, in contrast, significant declines in the inoculated pathogens were observed. Inoculated E. coli O157:H7 survived longer on kale (up to 19 d), in comparison, Campylobacter was undetectable by day 13 using enrichment and PCR or plating.


Funding for this work was provided in part, by the National Science Foundation award 2009717, and Virginia Agricultural Experiment Station and the Hatch Program of the National Institute of Food and Agriculture, U.S. Department of Agriculture.



University Libraries, Virginia Tech


Blacksburg, VA

Corresponding Author Name

Monica Ponder

Corresponding Author E-mail Address

Files/Folders in Dataset and Description

File 1 - The data for plate and PCR counts by media type over time File 2 - The temperature and humidity data over the course of the experiment