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Impact of surface topography on biofilm formation by Candida albicans

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posted on 24.02.2021, 13:28 by Katherine Lagree, Aaron Mitchell, William Ducker, Htwe Mon
Candida albicans is a fungal pathogen that causes serious biofilm-based infections. Here we have asked whether surface topography may affect C. albicans biofilm formation. We tested biofilm growth of the prototypical wild-type strain SC5314 on a series of polydimethylsiloxane (PDMS) solids. The surfaces were prepared with monolayer coatings of monodisperse spherical silica particles that were fused together into a film using silica menisci. The surface topography was varied by varying the diameter of the silica particles that were used to form the film. Biofilm formation was observed to be a strong function of particle size. In the particle size range 4.0–8.0 µm, there was much more biofilm than in the size range 0.5–2.0 µm. The behavior of a clinical isolate from a clade separate from SC5314, strain p76067, showed results similar to that of SC5314. Our results suggest that topographic coatings may be a promising approach to reduce C. albicans biofilm infections. The data set contains the image files obtained using confocal microscopy.

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University Libraries, Virginia Tech