36 files

Impact of surface topography on biofilm formation by Candida albicans

posted on 24.02.2021, 13:28 by Katherine Lagree, Aaron Mitchell, William Ducker, Htwe Mon
Candida albicans is a fungal pathogen that causes serious biofilm-based infections. Here we have asked whether surface topography may affect C. albicans biofilm formation. We tested biofilm growth of the prototypical wild-type strain SC5314 on a series of polydimethylsiloxane (PDMS) solids. The surfaces were prepared with monolayer coatings of monodisperse spherical silica particles that were fused together into a film using silica menisci. The surface topography was varied by varying the diameter of the silica particles that were used to form the film. Biofilm formation was observed to be a strong function of particle size. In the particle size range 4.0–8.0 µm, there was much more biofilm than in the size range 0.5–2.0 µm. The behavior of a clinical isolate from a clade separate from SC5314, strain p76067, showed results similar to that of SC5314. Our results suggest that topographic coatings may be a promising approach to reduce C. albicans biofilm infections. The data set contains the image files obtained using confocal microscopy.



University Libraries, Virginia Tech

Usage metrics

Virginia Polytechnic Institute and State University