"Effects of peripartal rumen-derived direct-fed microbial supplementation on lactation performance, metabolism, ruminal fermentation, and microbial abundance in dairy cows." Supplementing rumen-derived direct-fed microbial (DFM) can improve forage digestibility and ruminal VFA production and consequently improve milk production and feed efficiency. The DFM used in this study was composed of Clostridium beijerinckii (1 x 107), Pichia kudriavzevii (1 x 108), Ruminococcus bovis (1 x 108), Butyrivibrio fibrisolvens (1 x 108). Rumen-derived DFM promoted positive responses in lactation performance during the post-fresh period (6 - 14 wk postpartum), such as milk yield and feed efficiency.
Funding
The authors would like to thank Native Microbials, Inc. (San Diego,CA) for the financial support for this research.
The authors would like to thank the South Dakota Agricultural Experiment Station (Brookings, SD) for partially supporting this research.
Publisher
University Libraries, Virginia TechLocation
Blacksburg, VACorresponding Author Name
Johan OsorioFiles/Folders in Dataset and Description
[SupplementaryMaterialsEffectsPeripartalDairyCows.pdf]
- Supplementary TableS1. Frequency of occurrence of cows removed from the experiment fed a control diet (CON) or control diet plus direct-fed microbial (GF) from -21 +/- 5 d relative to calving through 100 DIM.
- Supplementary TableS2. Species-specific primers used in real-time qPCR assay for the quantification of selected rumen bacteria population.
- Supplementary FigureS1. Energy balance as % of requirements for all cows enrolled in this study. Energy balance for multiparous cows was calculated as NEB = [NEI/(NEM + NEMilk)] × 100, where NEI is NEL intake, NEM is NEL for maintenance, and MEmilk is NEL for milk synthesis. From 1- 5 wk, energy balance is incremental, while it plateaus after 6 wk. Energy balance for primiparous cows was calculated as NEB = [NEI/(NEM + NEMilk + NEG)] × 100.
- Supplementary FigureS2. Feed efficiency in terms of milk/DMI across parity in dairy cows fed a control diet (CON) or control diet plus a rumen-derived DFM (GF) from -21 ± 5 d relative to calving through 100 DIM. Mean separations between diets at a given time point were evaluated when at least a trend for a treatment × time (Trt × T) interaction (P ≤ 0.10) was observed, and differences (*) were declared at P ≤ 0.05. Values are means, and the standard errors are represented by vertical bars.
- Supplementary FigureS3. Ruminal butyrate (A) and caproate (B) in dairy cows fed a control diet (CON) or control diet plus a rumen-derived DFM (GF) from -21 ± 5 d relative to calving through 100 DIM. Mean separations between diets at a given time point were evaluated when at least a trend for a treatment × time (Trt × T) interaction (P ≤ 0.10) was observed, and differences (*) were declared at P ≤ 0.05. Values are means, and the standard errors are represented by vertical bars.
- Supplementary FigureS4. Ruminal butyrate in 2nd (A) and 3rd (B) lactation dairy cows fed a control diet (CON) or control diet plus a rumen-derived DFM (GF) from -21 ± 5 d relative to calving through 100 DIM. Mean separations between diets at a given time point were evaluated when at least a trend for a treatment × time (Trt × T) interaction (P ≤ 0.10) was observed, and differences (*) were declared at P ≤ 0.05. Values are means, and the standard errors are represented by vertical bars.
- Supplementary FigureS5. Blood glucose across 2nd and 3rd lactation dairy cows fed a control diet (CON) or control diet plus a rumen-derived DFM (GF) from -21 ± 5 d relative to calving through 100 DIM. Mean separations between diets at a given time point were evaluated when at least a trend for a treatment × time (Trt × T) interaction (P ≤ 0.10) was observed, and differences (*) were declared at P ≤ 0.05. Values are means, and the standard errors are represented by vertical bars.
- Supplementary FigureS6. Blood glucose (A), glutamic-oxaloacetic transferase (GOT; B), ceruloplasmin (C), haptoglobin (D), and reactive oxygen metabolites (E) in dairy cows fed a control diet (CON) or control diet plus a rumen-derived DFM (GF) from -21 ± 5 d relative to calving through 100 DIM. Values are means, and the standard errors are represented by vertical bars.
