dataset
posted on 2024-07-10, 18:34 authored by Alexis WestonAlexis Weston, T. Fernandes, M. De Oliveira, S Gaskin, T. Pilonero, M.D. Hanigan “Effects of isoleucine, lysine, valine, and a group of non-essential amino acids on mammary amino acid metabolism in lactating dairy cows.” Unbalanced dietary AA can affect AA transport by mammary glands. In the current work, infusions of Ile and Lys did not self-stimulate transport regulation nor affect net uptakes of other AA. Infusions of Ala, Gln, and Gly (AQG) increased mammary Tyr clearance rates but did not affect net uptakes of any AA. Ile, Lys, and AQG infusions did not affect milk protein production, but Val infusions decreased DMI, milk protein production, and mammary net uptakes of Leu, Lys, Met, and Na+-dependent transported AA. Val infusions limited nutrient supplies to the mammary glands and appeared to downregulate mammary AA transport.
Funding
This experiment was funded by USDA-NIFA Grant #2021-07170.
The authors would like to thank Ajinomoto (Itasca, IL, USA) for donating USP-grade L-Gln for use in this study.
History
Publisher
University Libraries, Virginia TechLocation
Blacksburg, VACorresponding Author Name
Mark HaniganFiles/Folders in Dataset and Description
[SupplementaryFigure1.pdf] - Supplementary Figure 1. Variability of CP concentrations in the TMR based on TMR tracker data from the last 8 d of each period and measured, pooled, feed DM and CP. The horizontal purple line represents the formulated TMR CP as a % of DM
[SupplementaryTable1.csv] - Supplementary Table 1. Citations for relevant Cumberland Valley analytical wet chemistry procedures for feed analyses
[SupplementaryTable2.csv] - Supplementary Table 2. Estimated and target metabolizable AA supplies and efficiencies from NASEM (2021) for each treatment utilizing measured DMI, milk production, milk components (protein and fat percentage), and BW. When applicable, AA infusions were inputted into the software as a jugular infusion in g/d. Arg target duodenal flows and efficiencies are not reported by NASEM (2021). Neither target or supplied duodenal flows and efficiencies are reported for NEAA by NASEM (2021)
[SupplementaryTable3.csv] - Supplementary Table 3. Data from Feedipedia and NASEM (2021) used to calculate jugular infusion rates for Ala, Gln, and Gly
[SupplenmentaryTable4.csv] - Supplementary Table 4. Milk and milk component production of primiparous lactating dairy cows during collection (d 14 of each period). Milk and protein production on d 14 were used to calculate mammary plasma flow
[SupplementaryTable5.csv] - Supplementary Table 5. Free AA concentrations in milk fluid of primiparous lactating dairy cows after 9-d of AA or saline (CON) jugular infusions
[SupplementaryTable6.csv] - Supplementary Table 6. Fatty-acid supply of base diet TMR estimated from the NASEM (2021) model and observed from d 8 until d 14 of each period for Group 1 in TMR tracker and wet chemistry laboratory results (Cumberland Valley Analytical Services)
[SupplementaryTable7.csv] - Supplementary Table 7. Amino acid concentrations in mammary venous plasma of primiparous lactating dairy cows after 9-d of AA or saline (CON) jugular infusions
[SupplementaryTable8.csv] - Supplementary Table 8. Arteriovenous difference of primiparous lactating dairy cows after 9-d of AA or saline (CON) jugular infusions
[References.pdf] - Citations from tables in the supplementary materials.