Ebraccatus_Inoculation_Experiment

The EIE was a short term study in which the eggs of hourglass treefrogs were inoculated with bacteria in different orders in order to test for the presence of priority effects in tadpole bacteria community development. To complement our tadpole experiment, a follow-up experiment was conducted in which broth containing wells within a well-plate were similarly inoculated with bacteria under two nutrient concentrations (Full LB broth or a 1:20 dilution LB broth). The sequences included are 16S rRNA sequences of the V4 variable region