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HoneyBee_FecalTetracycline_R2.fastq.gz (187.03 MB)
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HoneyBee_FecalTetracycline_metadata.csv (9.07 kB)
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ReadMe_HoneyBee_FecalTetracycline.txt (1.61 kB)
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HoneyBee_FecalTetracycline_R1.fastq.gz (2.18 GB)
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README.rtf (2.27 kB)
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Data associated with "Utilizing a novel fecal sampling method to examine resistance of the honey bee (Apis mellifera) gut microbiome to a low dose of tetracycline."

dataset
posted on 2024-09-27, 17:17 authored by Lisa BeldenLisa Belden, Casey GregoryCasey Gregory, Emma Bradford, Richard Fell, David HaakDavid Haak

These are bacterial 16S rRNA gene amplicon data and metadata from honey bee feces and guts; the dataset includes amplicon sequences, sequencing information necessary for demultiplexing and using the amplicon data, and tetracycline resistance gene (tetB and tetM) qPCR data for a subset of the samples.

This study comprised two parts. The first was a test to confirm that fecal samples from honey bees were a useful method to assess the bacterial gut community of honey bees. The second was a lab study using the fecal sampling method to investigate the impact of a low dose of tetracycline on resistance of the honey bee gut microbiome to disruption by antibiotics, focusing on responses of individual bees.

Funding

Collarborative Research: A systems approach to understanding signaling networks in host-microbiome-parasite interactions

Directorate for Biological Sciences

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History

Publisher

University Libraries, Virginia Tech

Location

Virginia Tech

Corresponding Author Name

Lisa Belden

Corresponding Author E-mail Address

belden@vt.edu

Files/Folders in Dataset and Description

[HoneyBee_FecalTetracycline_R1.fastq.gz]- forward (R1) bacterial 16S rRNA gene amplicon reads [HoneyBee_FecalTetracycline_R2.fastq.gz]- barcode (R2) bacterial 16S rRNA gene amplicon reads [HoneyBee_FecalTetracycline_metadata.csv]- includes all sample-associated metadata, including sequencing barcodes, and tetB and tetM qPCR data used in study. [ReadMe_HoneyBee_FecalTetracycline]- provides details of data columns in the HoneyBee_FecalTetracycline_metadata.csv file

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