Data associated with "Utilizing a novel fecal sampling method to examine resistance of the honey bee (Apis mellifera) gut microbiome to a low dose of tetracycline."
These are bacterial 16S rRNA gene amplicon data and metadata from honey bee feces and guts; the dataset includes amplicon sequences, sequencing information necessary for demultiplexing and using the amplicon data, and tetracycline resistance gene (tetB and tetM) qPCR data for a subset of the samples.
This study comprised two parts. The first was a test to confirm that fecal samples from honey bees were a useful method to assess the bacterial gut community of honey bees. The second was a lab study using the fecal sampling method to investigate the impact of a low dose of tetracycline on resistance of the honey bee gut microbiome to disruption by antibiotics, focusing on responses of individual bees.
Funding
Collarborative Research: A systems approach to understanding signaling networks in host-microbiome-parasite interactions
[HoneyBee_FecalTetracycline_R1.fastq.gz]- forward (R1) bacterial 16S rRNA gene amplicon reads
[HoneyBee_FecalTetracycline_R2.fastq.gz]- barcode (R2) bacterial 16S rRNA gene amplicon reads
[HoneyBee_FecalTetracycline_metadata.csv]- includes all sample-associated metadata, including sequencing barcodes, and tetB and tetM qPCR data used in study.
[ReadMe_HoneyBee_FecalTetracycline]- provides details of data columns in the HoneyBee_FecalTetracycline_metadata.csv file