Data associated with "Early life exposure to the antibiotic tetracycline alters in-hive development of the adult honey bee gut microbiome"

dataset

posted on 2024-12-20, 19:34 authored by Lisa BeldenLisa Belden, Casey GregoryCasey Gregory, Emma Bradford, Emma BuerenEmma Bueren, Korin JonesKorin Jones, Mychala SneadMychala Snead, Tiffany Duong, Olivia Sacci, Richard Fell, David HaakDavid Haak

These are bacterial 16S rRNA gene amplicon data and metadata from a honey bee experiment examining the impact of the antibiotic tetracycline on in-hive development of the gut microbiome. Honey bees were treated with tetracycline or control sucrose at emergence, marked by treatment, and then placed back in hives. Bees from treatment and control groups were then re-collected from hives at 7 and 14 days following treatment. The dataset includes bacterial 16S amplicon sequences, sequencing information necessary for demultiplexing and using the amplicon data, and total bacterial 16S qPCR data for a subset of the samples.

Funding

National Science Foundation BIO 1817736

History

Publisher

University Libraries, Virginia Tech

Location

Virginia Tech

Corresponding Author Name

Lisa Belden

Corresponding Author E-mail Address

belden@vt.edu

Files/Folders in Dataset and Description

[HoneyBee_InHiveA_R1.fastq.gz]- RunA forward (R1) bacterial 16S rRNA gene amplicon reads [HoneyBee_InHiveA_R2.fastq.gz]- RunA barcode (R2) bacterial 16S rRNA gene amplicon reads [HoneyBee_InHiveB_R1.fastq.gz]- RunB forward (R1) bacterial 16S rRNA gene amplicon reads [HoneyBee_InHiveB_R2.fastq.gz]- RunB barcode (R2) bacterial 16S rRNA gene amplicon reads [HoneyBee_InHiveA_metadata.csv]- Includes Illumina sequencing info for demultiplexing samples from the InHiveA sequencing run. [HoneyBee_InHiveB_metadata.csv]- Includes Illumina sequencing info for demultiplexing samples from the InHiveB sequencing run. [HoneyBee_InHive_ALL_metadata.csv]- includes all sample-associated metadata from both sequencing runs, merged into a single csv file, including sequencing barcodes, sequencing run information, and total 16S qPCR data used in study. [HoneyBee_InHive_Rcode.Rmd]- R code for analyses in associated manuscript. To use this code, the raw data (the sequencing files (R1) and (R2) plus the associated metadata files) needs to be first processed through the steps outlined in the manuscript in QIIME2 and R. ASV.csv and taxonomy.csv will be generated in the data processing steps. NEAmerged_Mappingfile.csv is the ALL metadata file that is in the data set. [ReadMe_HoneyBee_InHive_ALL]- provides details of data columns in the HoneyBee_InHive_ALL_metadata.csv file