Data and Code for "Cholesterol Modulates Membrane Elasticity via Unified Biophysical Laws"

<p dir="ltr">This data set contains data collected on lipid membranes consisting of phosphocholine lipids with varying amounts of cholesterol. The data files include:</p><ol><li>Neutron-spin echo (NSE) spectroscopy data on unilamellar vesicles for dynamic measurements of membrane bending fluctuations used to obtain the reported bending moduli.</li><li>Small-angle X-ray scattering (SAXS) data on unilamellar vesicles for measurements of structural membrane parameters used to obtain membrane thickness and lipid packing density.</li><li>Small-angle neutron scattering (SANS) data on unilamellar vesicles for measurements of structural membrane parameters used to obtain membrane thickness and lipid packing density.</li><li>Solid-state deuterium nuclear magnetic resonance (²H NMR) data on multilamellar lipid stacks for static measurements of the order parameter and dynamic measurements of the relaxation rates.</li><li>Trajectories for all-atom molecular dynamics (MD) simulations on all bilayers reported in this study (links to Zenodo repositories).</li><li>Code (.c and .py files) applied in fitting SAXS and SANS data in the form of a five-shell vesicle model (to be used as a plug-in model in the SasView data fitting package SasView 5.0.6), along with installation and data fitting instructions.</li><li>Code (.fdf file) applied in fitting the decays for NSE bending fluctuation spectra (to be used as a fitting function in Origin, Origin 2021b), along with installation and data fitting instructions.</li><li>Code (.m files) applied in processing and analyzing solid-state ²H NMR data (to be used as MATLAB scripts, MATLAB 2021a), along with installation and run instructions.</li></ol><p dir="ltr">This dataset is supplement to the article "Cholesterol Modulates Membrane Elasticity via Unified Biophysical Laws" published by the Nature Communications journal DOI: <a href="https://doi.org/10.1038/s41467-025-62106-0" rel="noreferrer" target="_blank">https://doi.org/10.1038/s41467-025-62106-0</a> by the following authors: Kumarage, Teshani; Gupta, Sudipta; Morris, Nicholas B.; Doole, Fathima T.; Scott, Haden L.; Stingaciu; Laura-Roxana; Pingali, Sai Venkatesh; Katsaras, John; Khelashvili, George; Doktorova, Milka; Brown, Michael; Ashkar, Rana</p>