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ws_cytb_reference.fna (1.23 kB)
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Lmp_2023.fasta (1.15 MB)
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Lmp2_2021.fasta (1.15 MB)
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Lmp1_2021.fasta (1.15 MB)
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EI_2021.fasta (1.15 MB)
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PB_2021.fasta (1.15 MB)
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README.md (3.79 kB)
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README.rtf (2.65 kB)
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Carcharodon carcharias CytB amplicon sequences from positive eDNA detections

Version 2 2024-09-27, 13:06
Version 1 2024-09-20, 16:38
dataset
posted on 2024-09-27, 13:06 authored by Francesco FerrettiFrancesco Ferretti, Brendan SheaBrendan Shea, Chiara Gambardella, Jeremy JenretteJeremy Jenrette, Stefano Moro, Khaled Echwikhi, Robert Schallert, Austin Gallagher, Barbara Block, Taylor Chapple

This dataset contains five DNA sequences that are PCR amplicons of the cytochrome b gene from the white shark (Carcharodon carcharias). The study aimed to detect this species using environmental DNA (eDNA) methods, targeting the cytochrome b gene due to its conservation within the mitochondrial genome and its divergence from the closely related Isurus oxyrinchus. The sequences display high similarity to the target species and marked differences from the relative species. These results suggest successful detection of C. carcharias through water sample collection, filtration, DNA extraction, PCR amplification, sequencing of the amplicons, and subsequent BLAST analysis.

Funding

The Augmentum Foundation

History

Publisher

University Libraries, Virginia Tech

Corresponding Author Name

Francesco Ferretti

Corresponding Author E-mail Address

ferretti@vt.edu

Files/Folders in Dataset and Description

Data File: ws_cytb_reference.fna Description: NCBI Reference Sequence: NC_022415.1 File: Lmp1_2021.fasta Description: sample, Lampedusa #1 2021 File: EI_2021.fasta Description: sample, Egadi Islands 2021 File: Lmp2_2021.fasta Description: sample, Lampedusa #2 2021 File: PB_2021.fasta Description: sample, Pantelleria Banks 2021 File: Lmp_2023.fasta Description: sample, Lampedusa 2023

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    Fish and Wildlife Conservation

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